셀루션 [Cellution]

‘SRRF-Stream+’ is a real time super-resolution microscopy functionality that super-charges the imaging performance of your standard microscope. Attain a level of resolution that was previously only possible from a dedicated high-end Super Resolution Microscope. Based on the “SRRF” technique (Super Resolution Radial Fluctuations), SRRF-Stream+ offers an easy and flexible software approach to super resolution.

NEW – now available for ZL41 Cell, Sona and selected Zyla 4.2 PLUS cameras making this amazing technology accessible to more applications at an even lower price point!

Enhanced Resolution – See beyond the diffraction barrier! Greater insights with resolutions down to ~100nm (2-6 fold improvement).
Real Time – Enhanced workflow, avoids post-processing. View in ‘Live Mode’.
Cell Friendly - Low Excitation Intensities (mW-W/cm2) prolong live cell observations and maintain accurate physiology.
Easy to use with conventional Fluorophores – Use your normal labelling protocols and labels like GFP.
Live Cell Dynamics – Full FOV super-res images every 1-2 secs with iXon EMCCD. > 10 fps using ROIs.
Cost-Effective – Convert conventional fluorescence microscopes to super-resolution microscopes.

Mitochondria

Image comparison of a fluorescently labelled BPAE cell, recorded with a widefield fluorescence microscope and a SRRF-Stream enabled iXon Life 888 EMCCD camera. A x63 objective was used, with further 2x magnification and 560nm illumination. 100 raw ‘input’ images were recorded for every resultant super-resolution image, resulting in a super-resolution image rate of 0.5 Hz. For a fair comparison without SRRF-Stream, 100 standard widefield images were recorded and then averaged. While the original image was of a larger field of cells, a zoomed ROI of one cell is shown here in order to more easily display a line intensity profile comparison through a small region. The improvement in resolving power is readily apparent.

SRRF vs SIM

HCV infected cells stained with anti-NS5A. Here we are comparing Widefield (WF), Structured Illumination Microscopy (SIM) and SRRF images (SRRF of the widefield image). The images are of the same field of cells, recorded on the same microscope, using identical objective and optical path. The only difference being that SIM was recorded using an sCMOS detector with 6.5µm pixels whereas the Widefield and resultant SRRF was recorded using an iXon EMCCD detector with 16 µm pixels. The superior resolving power of SRRF is evident, indicative that SRRF is achieving a greater than 2-fold improvement over the classical diffraction limit. SIM is theoretically limited to a 2-fold reduction of the classical diffraction limit. Sample courtesy of the Grove lab at UCL.

Clathrin Pits

Comparative images of Clathrin coated pits of live HeLa cells, labelled with mCherry, recorded on a widefield microscope at 2 FPS. 100 raw ‘input’ images were recorded for every resultant super-resolution image, resulting in a super-resolution image rate of 2 FPS. A line intensity profile is shown through a small region of the SRRF-Stream image, indicating resolution of structures that are 150nm apart. Sample preparation by Caron Jacobs (Ricardo Henriques and Mark Marsh labs at UCL).