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Andor Sona sCMOS


Now Even Faster and More Sensitive

Key Specifications


  • Sensitive: 95% peak QE 
  • Fast: up to 135 fps
  • Productive: up to 32 mm field of view 
  • Accurate: >99.7% linearity
  • Protected: UltraVacTM sensor enclosure
  • Longevity: 5-year vacuum warranty

Key Applications


  • Developmental biology 
  • Neuroimaging
  • Super-resolution 
  • Transcriptomics 
  • Intracellular trafficking 
  • Plasma membrane studies

Introducing Sona


The most sensitive back-illuminated sCMOS Cameras


Sona is Andor’s high performance, vacuum-cooled sCMOS camera platform, specifically for fluorescence microscopy. It has been designed from the ground up to extract the very best performance from the latest back-illuminated sCMOS sensors with 95% quantum efficiency.

1. Extended QE Response 

Back-illuminated sCMOS for highest possible photon collection. Capture every photon!

2. Market Leading Quantitative Accuracy 

Have confidence in your data with superb data fidelity:

P  >99.7% Linearity

P  <0.5% PRNU

3. True Imaging Flexibility

Select between 6.5 and 11 µm pixel options. Capture fast or weak signals with ease. Pre-set ROIs and flexible binning options.

4. Largest Imaging Area 

Up to 32 mm Field of View! Unique Antiglow technology lets you harness the full field of view without restrictions!

5. Camera based Super-resolution Capable 

Unlock real-time 

cell friendly super- resolution from your microscope with SRRF- Stream+!

6. Extended Dynamic Range (EDR)

One snap imaging captures full 16-bit image detail. Low-and high-level information in a single image!

7. Uncompromised High Speeds

Capture dynamic events faster than ever. New Sona-6 Extreme high speed mode accelerates speeds up to 135 fps!

8. Permanent Vacuum Seal Technology

The only back- illuminated sCMOS with permanent vacuum technology. Deepest cooling and longevity with 5 year seal warranty.•1

Sona-6 Extreme: Extreme Sensitivity & Speed


Sona-6 Extreme features a back-illuminated sensor with 95% QE and a 6.5 μm pixel size. This sensor format provides a perfect balance of sensitivity, speed, and resolution. Exceptionally flexible, and ideally suited to 40x and 60x magnification and today’s microscope port sizes. Compatible with SRRF-Stream+ Super-resolution.

Summary
Model4.2B-6
Sensor Size18.8 mm
Pixel Size6.5 μm
Quantum Efficiencyup to 95%
Read Noise1.0 e- (Low Noise, 12-bit)

1.6 e- (High Dynamic Range, 16-bit)

1.9 e- (High Speed, 11-bit

Dark Current0.1 e-/p/s
Max. Speed135 fps

NEW & IMPROVED Capture the dynamics of life with Sona-6 Extreme


  • Accelerated speeds: new high-speed mode accelerates speeds to 135 fps (full frame) via CoaXPress to capture the fastest cellular processes.
  • Improved sensitivity: the noise floor has been reduced by 25%. Sensitivity is thus improved for better detection of the weakest signals.
  • Enhanced image quality: PRNU has been reduced by 25% under low light conditions
  • Python ready - Updated camera SDK integrates a Python wrapper for speedy integration.

Intracellular Trafficking


Fast and sensitive imaging is crucial for studies of endosome cycling, Golgi vesicles pathways, axonal transport, hormone release or synaptic vesicle pool replenishment. 

Sona-6 Extreme with sensitivity, resolution and speed, is ideal for tracking intricate events and dependencies occurring within the cell’s vital transport and communications networks. 

Neuroimaging


Imaging of neurons and other specialised cells of the nervous system can be challenging for many detectors. Experiments can require high dynamic range or very sensitive detectors. Sona cameras have the required sensitivity and dynamic imaging capabilities for neuroimaging experiments. 

Developmental Biology


Imaging has been instrumental for following the entire lifespan of organisms to track fates of developing cells, tissues, and organs. Whole-embryo and whole-body imaging 

of well-established model organisms including the zebrafish and C. elegans 

can be captured in superb detail with the Sona-6 Extreme camera.

Plasma Membrane Dynamics


The plasma membrane can be imaged 

in many ways, which can involve direct membrane labelling with lipophilic or voltage sensitive dyes. Rapid remodelling of the plasma membrane can be imaged with 

the rapid frame rate, highly sensitive back- illuminated Sona cameras, perfectly suited to the low light conditions inherent to TIRF Microscopy. 

Super-resolution image of microtubules with B-TIRF. Image credit: F. Rivera-Molina, Yale University.
Super-resolution image of microtubules with B-TIRF. Image credit: F. Rivera-Molina, Yale University.
The early development of C. elegans, embryos labelled with GFP and mCherry, captured at 60x with Sona-6. Image Andor Technology.
The early development of C. elegans, embryos labelled with GFP and mCherry, captured at 60x with Sona-6. Image Andor Technology.
Organisation of neurons, astrocytes and glial cells within mouse brain, imaged with Sona at 40x. Image Andor Technology.
Organisation of neurons, astrocytes and glial cells within mouse brain, imaged with Sona at 40x. Image Andor Technology.

Sona-11: For the Widest Field of View


Sona-11 has the biggest sensor on the market with a full 32 mm sensor diagonal! Andor’s unique technology usefully accesses the entire 2048 x 2048 array offering 62% larger field of view than competing back-illuminated cameras. The 95% QE and larger pixel size of 11 µm provides optimal photon collection, for the most light-starved applications. Study structures and processes within the cell in perfect resolution using techniques such as confocal, TIRF and Single Molecule Localization Microscopy (SMLM).

Sona-11
Sona-11
Competitor sCMOS
Competitor sCMOS

See the full picture: With a 32 mm sensor diagonal Sona-11 has a field of view advantage:

  • 2.9x larger field of view vs typical sCMOS
  • 2.1x larger field of view vs 22 mm format sCMOS
  • 62% larger field of view vs competing back-illuminated sCMOS (1608x1608 array)
  • Capture weak signals – 95% QE is complemented by large 11 µm pixel size for optimal photon collection
  • SRRF-Stream+ Super-Resolution - Transform a standard microscope to super-resolution!
  • NEW Python ready - Updated camera SDK integrates a Python wrapper for speedy integration.
Summary
Model4.2B-112.0B-11
Sensor Size32 mm22 mm
Pixel Size11 x 11 μm
Quantum Efficiencyup to 95%
Read Noise1.6 e- median [1.8 e- rms]
Dark Current0.3 e-/p/s
Max. Speed48 fps70 fps

Single Molecule Imaging


Single molecule imaging experiments provide insights into processes that are not possible via normal ensemble imaging. Sona-11 is an alternative to EMCCD cameras when working with brighter labels and stronger signals. Sona-11 can provide significantly wider fields of view, higher speeds and exceptional dynamic range.

FCS


Sona-11 (32 mm) provides the best possible solution for many FCS experiments. The largest possible sensor area, high sensitivity and high speed are complemented by class leading linearity, which allow for the most accurate and precise measurements.

Transcriptomics


Detectors for such studies must have high sensitivity to help detection of the fluorescent RNA signal against the cell background. Large fields of view and high-speed are 

also important to maximise throughput of the image data using microarrays or tissue samples. Sona-11 (32 mm) is ideal for 

these studies with its combination of high sensitivity, speed and widest possible field of view.

Gene Editing


The best-in-class sensitivity offered by 

the back-illuminated deep cooled Sona sCMOS cameras are well suited to imaging of Crispr-Cas9 constructs, ideal for fast and sensitive detection of light emitted by labelled DNA/RNA or related proteins.

Mouse Fertilized eggs, Probe1: EGFP (EX/Em：488nm/525-50nm), Probe2: Kusabira Orange (Ex/Em: 561/600-50nm) Microscope: Ti2-E (Nikon), Objective: 40x /1.25 (Sil), Camera: Sona-11, Pinhole Size ：40 μm. Sample courtesy of Dr. Eiichi Okamura, Shiga University of Medical Science
Mouse Fertilized eggs, Probe1: EGFP (EX/Em：488nm/525-50nm), Probe2: Kusabira Orange (Ex/Em: 561/600-50nm) Microscope: Ti2-E (Nikon), Objective: 40x /1.25 (Sil), Camera: Sona-11, Pinhole Size ：40 μm. Sample courtesy of Dr. Eiichi Okamura, Shiga University of Medical Science
For the most demanding single molecule experiments EMCCD cameras are the most suitable. However, Sona-11 can be a viable option for stronger signals. Image from Andor Technology.
For the most demanding single molecule experiments EMCCD cameras are the most suitable. However, Sona-11 can be a viable option for stronger signals. Image from Andor Technology.
Decoded transcript locations of selected genes overlaid on stitched (n = 1 section per tissue). Scale bar, 100 μm. Split-FISH imaging repeated on at least one additional section per tissue, with similar results. Brain tissue showing differential localisation of transcripts in regions with (Itpr1) and without (Map4) cell bodies. (Goh et al., 2020)
Decoded transcript locations of selected genes overlaid on stitched (n = 1 section per tissue). Scale bar, 100 μm. Split-FISH imaging repeated on at least one additional section per tissue, with similar results. Brain tissue showing differential localisation of transcripts in regions with (Itpr1) and without (Map4) cell bodies. (Goh et al., 2020)

Super-Resolution Ready


SRRF-STREAM+


Want to enable fast and easy super-resolution down to 100 nm? Our exclusive camera-based technology enables super- resolution microscopy on conventional modern fluorescence microscopes in real-time. 

SRRF (Super-Resolution Radial Fluctuations), is a highly effective approach to super-resolution developed by the Henriques research group (Gustafsson et al., 2016). Andor’s unique and exclusive implementation of SRRF-Stream+ allows camera-based real-time super- resolution with low illumination intensities and normal fluorescent labels. 


  • Capture vibrant images – with stunning detail and high contrast.
  • Conventional fluorophores – simple labelling, no photo- switching required.
  • Cost-effective – convert conventional fluorescence microscopes to super-resolution microscopes.
  • Real time – enhanced workflow, avoids post-processing. View in ‘Live Mode’.
  • Low excitation intensities – prolonged live cell observations & accurate physiology.


STORM, PALM & DNA PAINT


The high sensitivity, low noise and high-speed capabilities of Sona are well suited to single molecule based ‘STORM / PALM’ and DNA PAINT approaches, revealing biological information down to ~10 nm.

“SRRF” super-resolution image taken with the Sona back- illuminated sCMOS camera, Andor Insight Awards, courtesy of Motosuke Tsutsumi, Research Institute for Electronic Science, Hokkaido University and National Institutes for Physiological Sciences, Aichi, Japan.
“SRRF” super-resolution image taken with the Sona back- illuminated sCMOS camera, Andor Insight Awards, courtesy of Motosuke Tsutsumi, Research Institute for Electronic Science, Hokkaido University and National Institutes for Physiological Sciences, Aichi, Japan.

Technical Data

ModelSona-11 (32 mm)Sona -11 (22 mm)Sona-6 Extreme
Sensor Type
Back-Illuminated Scientific CMOS
Array Size

2048 (W) x 2048 (H)

4.2 Megapixel

1400 (W) x 1400 (H)

2.0 Megapixel

2048 (W) x 2046 (H)

4.2 Megapixel

Pixel Size
11 x 11 μm6.5 x 6.5 μm
Image Area

22.5 mm x 22.5 mm 

(31.9 mm diagonal)

15.5 mm x 15.5 mm 

(21.8 mm diagonal)

13.3 mm x 13.3 mm 

(18.8 mm diagonal)

Readout Modes
Rolling Shutter
Pixel Readout Rates
100 MHz (High Dynamic Range, 16-bit)

200 MHz (Fast Speed, 12-bit)

180 MHz (Low Noise, 12-bit)

310 MHz (High Dynamic Range, 16-bit)

570 MHz (High Speed 11-bit)

 Quantum Efficiency
 up to 95%
Read Noise (e ) median
1.6 e- (at any readout rate)1.0 e  (Low Noise, 12-bit)

1.6 e- (High Dynamic Range, 16-bit)

1.9 e- (High Speed, 11-bit)

Sensor operating 

temperature•4

Air cooled

Water/liquid cooled

+15°C, -25°C

+15°C, -25°C, -45°C

+0°C, -25°C

+0°C, -25°C, -45°C

Dark Current

Air cooled (@-25°C )

Water/liquid cooled (@ 

-45°C)

0.7 e /pixel/s
0.3 e /pixel/s

 0.15 e-/pixel/s

 0.10 e /pixel/s

Active area pixel well depth
85000 e  (High Dynamic Range, 16-bit)
2600 e (Fast Speed, 12-bit, bit depth limited)
42000 e (High Dynamic Range, 16-bit)

1100 e- (Low Noise, 12-bit, bit depth 

limited)

1900 e (High Speed, 11-bit)

Dynamic Range
53000:1 (High Dynamic Range, 16-bit)
26250:1 (High Dynamic Range, 16-bit)
Data Range

16-bit (High Dynamic Range)

12-bit (Fast Speed)

16-bit (High Dynamic Range)

12-bit (Low Noise)

Linearity
> 99.7%
PRNU
< 0.5% (@ half-light range)
< 0.3%
Region of Interest (ROI)
User-definable, 1 pixel granularity, min. size 25 (w) x 1 (h)

User-definable, 1 pixel granularity, min. 

size 9 (w) x 1 (h)

Pre-defined ROI

1608 x 1608, 1200 x 1200, 1024 x 1024, 

512 x 512, 128 x 128

1024 x 1024, 

512 x 512, 

128 x 128

1608 x 1608, 1200 x 1200, 

1024 x 1024, 512 x 512, 128 x 128

Pixel Binning (on FPGA)
2 x 2, 3 x 3, 4 x 4, 8 x 8 (user-definable binning also available)
I/O
O: Fire Row 1, Fire Row n, Fire All, Fire Any, Arm, I: External
Trigger Modes
Internal, External, External Start, External Exposure, Software
Software Exposure Events
Start exposure - End exposure (row 1), Start exposure - End exposure (row n)
Image Timestamp Accuracy
25 ns
PC Interface
USB 3.0

USB 3.0•7 and  CoaXPress

Camera Window
AR coated UV grade fused silica window
Lens Mount
F-mount
C-mount


Quantum Efficiency
Quantum Efficiency

Frame Rates

Max Frame Rate (fps)

Sona-11 (32 mm)Sona -11 (22 mm)
ROI Size
(W x H)		16-bit

12-bit 

(Fast Speed)

16-bit

12-bit 

(Fast Speed)

2048 x 20482448--
1608 x 16083061--
1400 x 140035703570
1200 x 120041814181
1024 x 102448954895
512 x 5129519095190
256 x 256190378190378
128 x 128378750378750

Note: frame rates do not differ if partial or full rows are selected.


Sona-6 Extreme

Max Frame Rate (fps)

Low Noise Mode 

2-CMS (12-bit)

High Dynamic range 

Mode (16-bit)

High Speed Mode 

(11-bit)

ROI Size
(W x H)
USB
CXP
USB
CXP
USB
CXP
2048 x 2046
4344407440135
1024 x 1024
8787148148151270
512 x 512
174174295295532538
256 x 256
34634658758710461081
128 x 128
6866871165116620322125

Mechanical Drawings

Dimensions in mm [inches] (shown for C-mount)

Note: Support feet are recommended for mounting on microscope side ports. Adjustable support feet. Standard optical height 110 mm, TR-IXON- MNT-110.


Note: CoaXPress connection only available with SONA-4BV6X model or via CoaXPress upgrade. 


Weight: ~3 kg [6.61 lbs] approx.

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